Administrative data

Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
Study did not meet standard methods however, the study provides important biological information and is in a peer reviewed paper.

Data source

Reference
Reference Type:
publication
Title:
Effect of chlorine dioxide and metabolites on glutathione dependent system in rat, mouse and chicken blood.
Author:
Couri D and Abdel-Rahman MS
Year:
1980
Bibliographic source:
Journal of Environmental Pathology and Toxicology. 3: 451-460

Materials and methods

Methods:
in vivo
Principles of method if other than guideline:
The objective of the study is to provide information on the activity of glutathione reductase, glutathione peroxidase and catalase in mice from animals ddrinking various concentrations of ClO2 for 12 months: male Swiss-Webster mice were administered chlorine dioxide (1, 10, 100 or 1000 mg/L) in double distilled water for 20 hours/day, 7 days/week for 12 months in drinking water. After 12 months treatment, mice were sacrificed .

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):
yes
Test material identityopen allclose all
Identifier:
CAS number
Identity:
10049-04-4
Identifier:
EC number
Identity:
233-162-8
Identifier:
IUPAC name
Identity:
chlorous acid

Test animals

Species:
mouse
Strain:
Swiss Webster
Sex:
male
Details on test animals and environmental conditions:
Source: no data
Age: no data
Weight at study initiation: 15 to 17 g
Food consumption: ad libitum

Administration / exposure

Route of administration:
oral: drinking water
Vehicle:
other: double distilled water
Duration and frequency of treatment / exposure:
20 hours/day, 7 days/week, for 23 months (365 days)
Doses / concentrations:
1, 10, 100 and 1000 mg/L
No. of animals per sex per dose:
4 males/group
Control animals:
yes
Details on dosing and sampling:
After 12 months treatment, mice were sacrificed by decapitation and blood prepared for whole haemoglobine concentration and enzyme activity (glutathione peroxidase, catalase and glutathione reductaase). Methods of the assays were unstated.

Results and discussion

Bioaccessibility

Any other information on results incl. tables:
Effect of ClO2 on the activity was without relevant change. Glutathione peroxidase was significantly increased in the 1000 mg/L group (p<0.05), while in the 100 mg/L group, the activity was decreased (p<0.01). The activity of catalase was significantly higher in the 10, 100 and 1000 mg/L groups (p<0.01).

Applicant's summary and conclusion

Executive summary:
Effect of ClO2 on the activity was without relevant change. Glutathione peroxidase was significantly increased in the 1000 mg/L group (p<0.05), while in the 100 mg/L group, the activity was decreased (p<0.01). The activity of catalase was significantly higher in the 10, 100 and 1000 mg/L groups (p<0.01).