Short-term toxicity to aquatic invertebrates.010

Short-term toxicity to aquatic invertebrates.010

Administrative data

Purpose flag:

supporting study

Study result type:

read-across from supporting substance (structural analogue or surrogate)

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

No GLP statement or analysis

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Materials and methods

Test guideline

Qualifier:

according to

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

yes

Remarks:

3 concentrations instead of 5 were tested

Principles of method if other than guideline:

The acute toxicity of sodium chlorite to Daphnia magna in a 48 hr static test was assessed.

GLP compliance:

no

Test materials

Identity of test material same as for substance defined in section 1 (if not read-across):

no

Test material identityopen allclose all

Test material identity 1

Identifier:

CAS number

Identity:

7758-19-2

Test material identity 2

Identifier:

EC number

Identity:

231-836-6

Test material identity 3

Identifier:

IUPAC name

Identity:

sodium chlorite

Details on test material:

- Name of test material (as cited in study report): sodium chlorite
- Molecular formula (if other than submission substance): no data
- Molecular weight (if other than submission substance): no data
- Smiles notation (if other than submission substance): no data
- InChl (if other than submission substance): not applicable
- Structural formula attached as image file (if other than submission substance): no data
- Substance type: no data
- Physical state: Colourless liquid.
- Analytical purity: 31.12% (w/w) in purified water
- Impurities (identity and concentrations):
- Composition of test material, percentage of components: no data
- Isomers composition: no data
- Purity test date:
- Lot/batch No.: 6624
- Expiration date of the lot/batch: no data
- Radiochemical purity (if radiolabelling):
- Stability under test conditions: no data
- Storage condition of test material: at room temperature and protected from the light

Details on properties of test surrogate or analogue material:

no data

Analytical monitoring:

no

Details on sampling:

Water quality parameters were tested, namely temperature, hardness, dissolved oxygen and pH at 0 hr and 48 hr for the control and highest concentration.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Tests solutions were prepared by further dilution of the stock solution with reconstituted water, or directly from the test item. 100 mg/l of test item solution plus 2 other range-finding conc. were prepared.
- Eluate: Water. pH of this solution = 8.0 +-0.5 and the sum of Ca and Mg ions on the solution is 2.5 mmol/l with a molar ratio of Ca/Mg = 4:1 and of Na/K = 10:1
- Differential loading: at least 10 ml of test solutions were provided for each animal
- Controls: no data
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): not applicable 
- Concentration of vehicle in test medium (stock solution and final test solution): not applicable
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Strain: clone 5
- Source: CIT
- Age at study initiation (mean and range, SD): between 6 to 24 hours
- Weight at study initiation (mean and range, SD): no data
- Length at study initiation (length definition, mean, range and SD): no data
- Method of breeding:
- Feeding during test: NO
- Food type:
- Amount: 
- Frequency:

ACCLIMATION
- Acclimation period: none
- Acclimation conditions (same as test or not): not applicable because there was no acclimation
- Type and amount of food: 0.1-0.2 mg of carbon per day per Daphnia, in the form of the algal strain Scenedesmus subspicatus (cultured at CIT)
- Feeding frequency: no data
- Health during acclimation (any mortality observed):

QUARANTINE (wild caught)
- Duration: no data
- Health/mortality: no data

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

306 mg/L

Test temperature:

19.6 °C

pH:

7.91 - 8.45

Dissolved oxygen:

6.7 - 9.3 mg/L

Nominal and measured concentrations:

Nominal concentrations:
1, 10 and 100 mg/L (expressed as active component)
(Doses are equivalent to 0.75, 7.5 and 75 mg chlorite/L)

Details on test conditions:

TEST SYSTEM
- Test vessel: no data
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: no data
- Aeration: none
- Type of flow-through (e.g. peristaltic or proportional diluter): no data
- Renewal rate of test solution (frequency/flow rate): no
- No. of organisms per vessel: 20
- No. of vessels per concentration (replicates): 4 
- No. of vessels per control (replicates): 4 
- No. of vessels per vehicle control (replicates): not applicable
- Biomass loading rate: no data

OTHER TEST CONDITIONS
- Adjustment of pH: with HCl or NaOH before the addition of animals
- Photoperiod: 16:8
- Light intensity: no data

Reference substance (positive control):

no

Results and discussion

Effect concentrationsopen allclose all

Effect concentrations 1

Duration:

48 h

Endpoint:

EC50

Effect conc.:

< 1 other: mg sodium chlorite/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

48 h

Endpoint:

EC50

Effect conc.:

< 0.75 other: mg chlorite/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

no data

Results with reference substance:

no data

Reported statistics and error estimates:

no reports

Any other information on results incl. tables:

Table A7_4_1_2 (3)-6:       Cumulative immobilisation data

Test Substance Concentration (nominal) 1 [mg sodium chlorite/L]
	Immobile Daphnia
	Number     24 h          48 h		Percentage      24 h         48 h
0	0/5	0/5	0	0
	0/5	0/5	0	0
	0/5	0/5	0	0
	0/5/	0/5	0	0
1	4/5	5/5	80	100
	4/5	5/5	80	100
	1/5	5/5	20	100
	3/5	5/5	60	100
10	2/5	5/5	40	100
	4/5	5/5	80	100
	3/5	5/5	60	100
	3/5	5/5	60	100
100	5/5	5/5	100	100
	5/5	5/5	100	100
	5/5	5/5	100	100
	5/5	5/5	100	100

1 Concentrations were nominal

Table A7_4_1_2 (3)-7:       Effect data

	EC501	95 % c.l.	EC01	EC1001
24 h [mg sodium chlorite/L]	Not specified	Not specified	Not specified	Not specified
48 h [mg sodium chlorite/L]	1 mg/L (n)	Not specified	Not specified	7 mg/L

1 effect data are based on nominal (n) concentrations

Applicant's summary and conclusion

Conclusions:

The 48 hr EC50 < 1 mg sodium chlorite/L (equivalent to 0.75 mg chlorite/L).

Executive summary:

Daphnia magna were exposed to 3 different sodium chlorite concentrations (1, 10, and 100 mg/L), following OECD Guideline 202 (with a restriction due to the fact that 3 concentrations were tested instead of 5 as recommended). Results obtained demonstrate that EC50 (48h) < 1 mg/L of Sodium chlorite.